Journal: Cancer Biology & Therapy

Article Title: The v8-10 variant isoform of CD44 is selectively expressed in the normal human colonic stem cell niche and frequently is overexpressed in colon carcinomas during tumor development

doi: 10.1080/15384047.2023.2195363

Figure Lengend Snippet: Exon map of human CD44 isoforms showing regions of rabbit genomic antibody (RGA) binding and immunohistochemical (IHC) reactivity . The anti-CD44 rabbit genomic antibodies were generated to react to 16 different epitopes that span the entire length of the CD44 molecule. Antibody 4077, which binds to CD44’s amino-portion, will react to all forms of CD44 including the standard CD44 isoform and all variant CD44 isoforms except CD44v2. Antibody 4080 will react to CD44v6+ cells and antibody 4081 will recognize CD44v8–10+ cells. Exon numbering is based on Screaton et al. and Uniprot .

Article Snippet: Rabbit anti-human CD44 genomic antibodies were generated at Strategic Diagnostics Inc. (SDIX, Newark, DE) using a proprietary technology developed at SDIX .

Techniques: Binding Assay, Immunohistochemical staining, Generated, Variant Assay

Journal: Cancer Biology & Therapy

Article Title: The v8-10 variant isoform of CD44 is selectively expressed in the normal human colonic stem cell niche and frequently is overexpressed in colon carcinomas during tumor development

doi: 10.1080/15384047.2023.2195363

Figure Lengend Snippet: Summary of screening results on the panel of anti-CD44 rabbit genomic antibodies . Flow cytometric analyses showed that four antibodies (1681, 4077, 4081, 4083) had strong reactivity to their corresponding antigens (Figure S2). Sandwich ELISA immunoassays showed that five antibodies (4077–4081) had reactivity to antigens. IHC staining was used to screen reactivity against matched normal versus malignant colonic tissue samples. We found that: i ) Three antibodies (4076, 4077, 4083) stained both normal colonic epithelial and colon carcinoma tissues; ii ) Three antibodies (4081, 4082, 4085) strongly stained colon carcinomas, but did not stain normal colonic epithelium; iii ). One antibody (4080) stained normal colonic epithelium, but not colon carcinoma. GA = genomic antibody, Seq = AA sequence, #aa = number of amino acids, AP = affinity purified, Tx Pr = direct bind ELISA, IHC = immunohistochemistry, Flow = flow cytometry, IA = immunoassay

Article Snippet: Rabbit anti-human CD44 genomic antibodies were generated at Strategic Diagnostics Inc. (SDIX, Newark, DE) using a proprietary technology developed at SDIX .

Techniques: Sandwich ELISA, Immunohistochemistry, Staining, Sequencing, Affinity Purification, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Cancer Biology & Therapy

Article Title: The v8-10 variant isoform of CD44 is selectively expressed in the normal human colonic stem cell niche and frequently is overexpressed in colon carcinomas during tumor development

doi: 10.1080/15384047.2023.2195363

Figure Lengend Snippet: Bioinformatics analysis of CD44's ability to predict CRC patient survival. Bioinformatics results on analysis of CD44 expression levels and CRC patient survival (n = 597) was based on RNA-seq data generated by The Cancer Genome Atlas (TCGA) project. Note that, in the TCGA database, CD44 expression encompasses all forms of CD44 including standard CD44 and all CD44 variant isoforms [56] so it doesn't distinguish between CD44 isoforms. The Kaplan-Meier plot shows that CD44 expression correlates with CRC patient survival (p < 0.05).

Article Snippet: Rabbit anti-human CD44 genomic antibodies were generated at Strategic Diagnostics Inc. (SDIX, Newark, DE) using a proprietary technology developed at SDIX .

Techniques: Expressing, RNA Sequencing, Generated, Variant Assay